Cloning and characterization of four novel SnRK2 genes from Triticum polonicum

Cloning and characterization of four novel SnRK2 genes from Triticum polonicum

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SnRK2 are plant-specific serine/threonine kinases that are involved in plant responses to read more abiotic stresses.In this study, four novel genes SnRK2s:TpSnRK2.11, TpSnRK2.2, TpSnRK2.

5, and TpSnRK2.10 from dwarf Polish wheat (Triticum polonicum L.) were characterized and classified into three groups.TpSnRK2.

5 and TpSnRK2.11 were members of group 1; TpSnRK2.2 was member of group 2; TpSnRK2.10 belonged to group 3.

The expression of TpSNRK2.2 was strongly regulated by polyethylene glycol (PEG), NaCl, and cold in roots and leaves, as well as by ABA in leaves.The transcript of TpSnRK2.5 was intensely induced by all the here treatments in roots and leaves.

The distinct expression patterns of TpSnRK2.10 indicate that this gene was very sensitive to ABA and NaCl, less sensitive to cold and PEG.The transcript of TpSnRK2.11 was activated significantly by PEG, NaCl, and cold, but weakly by ABA.

Our results indicate that these four genes were probably involved in wheat responses to different abiotic stresses in different tissues.